b'COVER FEATUREdegree of specificity is powerful because it means survey results are very accurate and notconfoundedbydetectingnon-target species by mistake.InStep2,theconsultantdevelopsa suitable field sampling regime that targets theecologyofthespeciesofinterest. Consideringthespeciesrange,habitat requirementsandbehaviourmeans strategic decisions by the ecologist can be made regarding where and how many water samplesshouldbetaken,aswellasthe sample volume. If surveying for bats, guano pellets, hair from maternity roost sites, soil, or even water samples from a nearby pond wherebatsfeedonflyinginsectscanbe collected as sources of bat eDNA.Step3involvescollectingapredeter-minednumberandvolumeofwater samplesfromvariouslocationsontheCollection of water samples: pictured here is a property.Eachsampleisfilteredusingfilter cartridge used to capture floating eDNAaspecialpumpandfiltercartridgeto capturethefloatingeDNAoutofthe water samples. To minimize risk of eDNA degradation during sample transport back to the lab, its best to extract and preserve the eDNA from the filters immediately in the field. Extracting and preserving eDNA fromwatercanbedoneinthefieldin minutes. Other samples (e.g., guano, hair or soil) are transported back to the lab for extractionpriortogeneticanalysis.All samplesshouldbestoredinacoolerat around 4C during transport.InStep4,eachextractedsampleis quantifiedtomeasurethetotalamount of eDNA in the sample (including target andnon-targeteDNA)andanalyzedin triplicateusingquantitativereal-time polymerase chain reaction technology to see if the target species eDNA is present Turtle eDNA sampling in action: extracting and preservinginthesamples.Theresultsandtheir eDNA from water can be done in the field in minutes importance are interpreted in the context Greycoats Land and Environment Management System Greycoats Land and Environment ManagementStore and easily review Systeman online digital filing cabinet site licenses, permits, designed for the information requirements certifications, of the aggregate, asphalt, Ready-Mix, and significant dates, andGreycoat Quarry cement industry. ID: Greycoat Quarryother documents linked Parent: Greycoat AggregatesMap based design for all your sites. View Site Site Documentsto your operations.Full access from anywhere with internet.Advanced security & password protection.Contact Greycoat Software for details and to access our online demo. sales@greycoat.ca 519-746-6666greycoat.ca@GreycoatSW 8 AVENUESFALL 2020'